Download Rotor Gene 6000 Software Testing
Getting Started on the Rotor-Gene-6000 Jennifer McMahon, PhD corbett LIFE SCIENCE the world’s only real-time rotary thermo-optical analyser Turning to instrument design, this brings us to the Rotor-Gene, the worlds only centrifugal thermal analyser. Here we’re looking top-down at the open lid of the Rotor-Gene showing the rotor in position in the reaction chamber. If you look carefully you’ll see tubes arranged under the silver locking ring in a circular configuration, similar to a centrifuge. Cross-section of rotary optics Reaction Chamber Detection Filters Lens PMT Detector Assembly Spindle/Motor Assembly Tubes Spin in Rotor (Red) LED Light Source Assembly To better understand centrifugal design, let’s look at an x-ray cross section view through the middle of the RG. Click 1: Firstly, here’s the reaction chamber, a light-sealed unit, the top half of which swings open with the RG lid. Click 2: Now here’s the rotor in position (here colored red). This is a 36-well rotor holding the largest tube type (0.2 mL).
This makes it easier to see the individual tubes. Click 3: This allows the tubes to spin around and pass the optic subsystem, which we’ll look in a moment. Click 4: The rotor doesn’t hang in mid air as shown, it sits on a motorized spindle assembly, just like in a centrifuge. Click 5: The light source is an array of individual LEDs (light-emitting diodes) arranges in a “gattler” assembly. The gattler turns to allow individual LEDs to be used as appropriate. There are up to 6 LEDs depending on the model.
Click 6: Detection of optical signals is via a photomultiplier (PMT), positioned at the side of the unit. Click 7: The Detection subsystem contains a lens Click 8: And a set of up to 6 filters, depending on the model.
Click 9: To detect signals, the selected LED illuminates each reaction as it spins past with a high-power burst of light. Click 10: The reaction emits fluorescent energy Click 11: which is focussed through the lens, passes through the selected filter and is detected by the PMT and reported. It’s important to note that each tube is moved to the identical optical pathway. This simply means that there is no optical variation at each tube position. This is why the RG does not require any optical callibration, as other systems do. Click 12: The RG is heated and cooled by fast-moving air.
Even when collecting fluorescent signals the air in the chamber is circulating around each tube as they spin past the detector. This ensures well-to-well thermal variation is absolutely minimal—below detectable levels in fact.
Contrast this with other systems that typically suffer one degree or more difference across the block, particularly in edge wells. To complete our understanding of the mechanism, vents are opened and cool air is imported through the base of the chamber and hot air is expelled through the top of the chamber (and out the vents on the lid) when the instrument is in cooling mode (not shown) When heating, the chamber seals and an overhead heating element (not shown) raises the temperature of circulating air.
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MTAS© Optical Calibration System Date: 04-Nov-2014 CYCLERtest introduces its new hard and software for Optical Calibration of qPCR cyclers and HRM modules. QPCR thermocycler Optical Calibration & HRM (High Resolution Melt) modules. Traceable. Multichannel. All Dyes ranging from 440 to 650 nm.
Cq detection and calibration. Tm detection and calibration. Tm validation (ratio Tm or RTm). Linear Sensitivity Factor (LSF). Channel Peak Height Consistency (CPHC). Identical Fragment Indicator.
Full Certificate Document. Patented by CYCLERtest Free Optical Calibration for Benelux customers up to December 15th, 2014 if combined with CYCLERtest's ISO17025 accredited Temperature thermocycler calibration services. Download an example certificate For more information click. ISO17025 accredited Calibration now also for Rotor-Gene® Date: 20-Aug-2013 On site calibration for Qiagen® Rotor-Gene® systems models 6000 and Q Rotor-Genes under ISO17025 accreditation is added to our service portfolio. CYCLERtest, an ISO17025 accredited company calibrating (q)PCR cyclers, has designed and developed this new MTAS® hardware and software tool enabling Rotor-Gene® Calibration. This new wireless system consists of a 12 sensor-equipped 72 Rotor with incorporated RF communication hardware. The system and the service are ISO17025 calibrated and accredited.
Service is available from September 1st, 2013 and can be combined with other (q)PCR cycler calibrations, regardless of brand or model of the cycler. All calibrations are under ISO17025 and comply to all regulations, including but not limited to GLP, GMP, ISO15189, ISO13485, CAP, EFI, CLIA, CPA, NY State, FDA, NFSCT, AAVLD, OECD, RiLiBAEK, Food Diagnostics and others. Click to request your on site Rotor-Gene® calibration Click to request a calibration quote Download an example Calibration Certificate Download CYCLERtest ISO17025 certificate Download CYCLERtest ISO17025 calibration scope.
Normalizing SOPs Date: 24-Jul-2009 Many laboratories have SOP (Standard Operation Procedures) in place. Incorporation of new components in SOP’s are generally labor and time consuming. In the (q)PCR process, variables as cyclers, plastic disposables, kit components and work procedures are typically described and incorporated in SOP’s. Often different SOP’s in place for one application are used on several (different) cyclers. BIOplastics and CYCLERtest® have formulated a strategy which enables exclusion of the cycler as well as the used disposable variable. This strategy actually provides users the ability to normalyze their SOP.
Rotor Gene Q Software Download
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